The Botany Libraries offer service in two reading rooms. The main reading room is located on the second floor of the Harvard University Herbaria (HUH). It is open Monday through Friday from 9:00 am until 5:00 pm except for University holidays.

The Farlow Library reading room is located in the Farlow Herbarium which is accessed via the first floor of HUH.  The reading room is open Monday through from 9:30 am until noon except for University holidays.

Archival collections may be consulted by appointment only on Monday through Friday from 10:00 am until 4:00 pm.